α kg  (MedChemExpress)

Journal: Molecular Biomedicine

Article Title: Macrophage Trem2 deficiency aggravates aging-induced vascular remodeling by acting as a non-classical receptor of interleukin-13

doi: 10.1186/s43556-025-00377-1

Figure Lengend Snippet: IL-13/Trem2 combination enhance the beneficial crosstalk between macrophage and VSMCs through Syk-Sp1-SLC25A51 pathway-mediated upregulation of α-KG. a Measuring the α-KG concentration in senescent macrophages upon Ang II stimulation at different time points with/without IL-13 treatment. n = 5 samples/group. b Measuring the α-KG concentration in IL-13-treated senescent macrophages isolated from WT and T2-KO mice. n = 5 samples per group. c Measuring the concentration of α-KG in senescent macrophages treated with IL-13 and then transfected with a Trem2-overexpressing lentivirus. n = 5 samples per group. d Measurement of the α-KG concentration in IL-13-treated T2-KO senescent macrophages transfected a lentivirus for Sp1 or SLC25A51 overexpression. n = 5 samples per group. e Measuring the concentration of α-KG in IL-13-treated T2-KO senescent macrophages transfected a lentivirus for Sirt2, Sirt3, Sirt5, or Nampt overexpression. n = 5 samples/group. f QPCR quantification of contractile protein markers Cnn1, SM-22α, and α-SMA in α-KG-treated senescent VSMCs. n = 6 samples/group. g Measurement of Caspase 3/7 activity in α-KG-treated senescent VSMCs. n = 5 samples per group. h , i DHE staining images and quantification in α-KG-treated senescent VSMCs. Scale bar: 100 µm. n = 5 samples per group. j, k Images and quantification of the wound-healing assay regarding the effects of α-KG on VSMC migration in a co-culture experimental system of IL-13-treated T2-KO senescent macrophages and VSMCs. Scale bar: 100 µm. n = 6 samples per group. l QPCR quantification of the effect of α-KG on contractile protein markers α-SMA in a co-culture experimental system of IL-13-treated T2-KO senescent macrophages and VSMCs. n = 5 samples per group. m, n Immunofluorescence staining and quantification of the effect of α-KG on contractile protein markers α-SMA in a co-culture experimental system of IL-13-treated T2-KO senescent macrophages and VSMCs. Scale bar: 100 µm. n = 5 samples per group. o, p Edu staining images and quantification regarding the effect of α-KG on cell proliferation of VSMCs in a co-culture experimental system of IL-13-treated T2-KO senescent macrophages and VSMCs. Scale bar: 100 µm. n = 5 samples per group. q, r DHE staining images and quantification data regarding the effect of α-KG on cell proliferation of VSMCs in a co-culture experimental system of IL-13-treated T2-KO senescent macrophages and VSMCs. Scale bar: 100 µm. n = 5 samples per group. s, t Caspase 3/7 activity images and quantification data regarding the effect of α-KG on cell apoptosis of VSMCs in a co-culture experimental system of IL-13-treated T2-KO senescent macrophages and VSMCs. Scale bar is 100 µm. n = 5 samples per group. * p < 0.05, ** p < 0.01

Article Snippet: To evaluate the efficacy of α-ketoglutarate (α-KG) supplementation in modulating vascular aging in vivo, 18-month-old male WT and T2-cKO mice were randomly allocated into four experimental groups and provided free access to either standard chow or chow supplemented with 2% α-KG (MedChemExpress, 328–50–7) for six months.

Techniques: Concentration Assay, Isolation, Transfection, Over Expression, Activity Assay, Staining, Wound Healing Assay, Migration, Co-Culture Assay, Immunofluorescence

Journal: Molecular Biomedicine

Article Title: Macrophage Trem2 deficiency aggravates aging-induced vascular remodeling by acting as a non-classical receptor of interleukin-13

doi: 10.1186/s43556-025-00377-1

Figure Lengend Snippet: Administration of α-KG counteracts the vascular ageing and dysfunction triggered by macrophage Trem2 loss in vivo. a PWV measurements in four groups of aged mice. n = 8 samples/group. b Measurement of arterial vessel contractions mediated by PE in four groups of aged mice. n = 8 samples per group. c Measurement of arterial vessel relaxation mediated by SNP in four groups of aged mice. n = 8 samples per group. d H&E staining analysis on aortic samples from four groups of aged mice. Scale bar: 100 µm. n = 8 samples per group. e, f Masson staining images and quantification of aortic samples from aged mice. Scale bar: 100 µm. n = 8 samples per group. g, h DHE staining images and quantification on aortic samples from aged mice. Scale bar: 100 µm. n = 8 samples per group. i-l QPCR quantification of the expressions of Collagen I, α-SMA, IL-6 and Sirt6 in aortic samples from aged mice. n = 5 samples per group. m Working model: The macrophage IL-13/Trem2/Syk-Sp1-SLC25A51 axis mitigates vascular aging by enhancing mitochondrial NAD + transport and α-ketoglutarate (α-KG) production. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: To evaluate the efficacy of α-ketoglutarate (α-KG) supplementation in modulating vascular aging in vivo, 18-month-old male WT and T2-cKO mice were randomly allocated into four experimental groups and provided free access to either standard chow or chow supplemented with 2% α-KG (MedChemExpress, 328–50–7) for six months.

Techniques: In Vivo, Staining